HAEMATOLOGICAL INDICES AND WEIGHT EFFECT IN FEMALE WISTAR RATS TREATED WITH DOSE-DEPENDENT AQUEOUS EXTRACT OF Raphia hookeri FRUIT MESOCARP
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Abstract
Background: Research aimed to evaluate haematological indices and weight effects in female wistar rats treated with dose-dependent aqueous Extract of Raphia Hookeri Fruit Mesocarp. Raphia hookeri and its therapeutic potential warranted this scientific investigation.
Methods: 28 female Wistar rats weighing 130g to 200g, were grouped into 4 groups, Group 1 as control fed with feed and water, Group 2 received 1000 mg/kg, Group 3, 2000 mg/kg, and Group 4 3000 mg/kg body weight daily for 28 days. Animals were sacrificed after the treatment period, blood samples were collected, and erythrocyte count, MCV, MCH, MCHC, and platelet counts were analyzed using standard haematological techniques. Statistical analysis was conducted with SPSS version 21.0, and results were expressed mean ± standard error of the mean (SEM), p<0.05 considered statistically significant.
Results: Results showed erythrocyte counts of 5.80 ± 0.12 in control group, a significant decrease in treated groups 2, 3 and 4. MCV levels increased significantly across treatment groups, control at 72.00 ± 1.73, compared to Groups 2, 3, and 4, respectively. MCHC values decreased significantly in treated groups, 412.00 ± 4.04 for control. Reduced TWBC values for group 1 to 4, Neutrophil for group 1 to 4, Lymphocytes for group 1 to 4, Eosinophils for group 1 to 4 and Monocytes for group 1 to 4. Platelet counts showed dose-dependent decrease, 250.00 ± 1.15 in control and significantly lower counts of 120.00 ± 2.89, 100.00 ± 1.73, and 94.00 ± 2.31 in Groups 2, 3, and 4, respectively.
Conclusion: Higher doses may significantly impact erythrocyte leukocytes and platelet parameters, suggesting potential haematological risks as such moderation is recommended.
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